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INHIBITION OF BIOFILM PRODUCING GRAM NEGATIVE CLINICAL ISOLATES AND THEIR ANTIBIOGRAM PATTERN
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  • Ojaswee Shrestha,
  • Nabina Shrestha,
  • Sadhana Khanal,
  • Sushant Pokhrel,
  • Sujina Maharjan,
  • Tika Bdr Thapa,
  • Puspa Raj Khanal,
  • Govardhan Joshi
Ojaswee Shrestha
Manmohan Memorial Institute of Health Sciences
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Nabina Shrestha
Manmohan Memorial Institute of Health Sciences
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Sadhana Khanal
Manmohan Memorial Institute of Health Sciences
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Sushant Pokhrel
Manmohan Memorial Institute of Health Sciences
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Sujina Maharjan
Manmohan Memorial Institute of Health Sciences
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Tika Bdr Thapa
Manmohan Memorial Institute of Health Sciences
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Puspa Raj Khanal
Sumeru Hospital Pvt. Ltd.
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Govardhan Joshi
Manmohan Memorial Institute of Health Sciences
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Abstract

Background: Bacterial biofilm is a major virulence factor that posses a threat to patients leading to chronic infections. Therefore, it is crucial to identify biofilm production as well as their inhibition and reduction. This study was an attempt to investigate biofilm production among gram-negative isolates and assessment of inhibitory and reduction potential of EDTA and DMSO towards them and also observe the antimicrobial resistance pattern among biofilm producers and biofilm non-producer. Methods: Isolation and identification of bacterial isolates were performed by standard microbiological methodology. The antibiotic susceptibility pattern was determined by the Kirby Bauer disk diffusion method and β-lactamases by the combination disk method. Biofilm formation was detected through Tissue Culture Plate(TCP) method, and different concentrations of EDTA and DMSO were used to determine their inhibitory and reduction property against biofilm. Both inhibition and reduction by the various concentration of EDTA and DMSO were analyzed using paired t-test. Results: Among the 110 clinical isolates 61.8% were found to be Multidrug resistance(MDR) with the 33 (30%) produced Extended-spectrum β-lactamases(ESBL), 16 (14.5%)Metalloβ-lactamases(MBL) and 9 (8%)Klebsiella pneumonia carbapenemase(KPC). Biofilm formation was detected in 35.4% of isolates. Biofilm producing organisms showed antibiotics resistance to Cephalosporins, Chloramphenicol, Gentamycin, and Carbapenem. The inhibition and reduction of biofilm were significantly lower (p<0.05) for 1mM of EDTA and 2% of DMSO. Conclusions: EDTA and DMSO were found to possess potential activity against biofilm. Hence, EDTA and DMSO might be used invitro as an effective antibiofilm agent to control the biofilm-associated infection and for a possible therapeutic approach.

Peer review status:UNDER REVIEW

02 Jan 2021Submitted to International Journal of Clinical Practice
22 Feb 2021Assigned to Editor
22 Feb 2021Submission Checks Completed
23 Feb 2021Reviewer(s) Assigned
25 Feb 2021Review(s) Completed, Editorial Evaluation Pending